특허 및 연구성과

Abstract: Stauntonia hexaphylla (Thunb.) Decne (SH) is known for its anti-inflammatory,

analgesic, antioxidant, and anti-osteoporotic properties. This study investigated the composition

of SH leaves and fruits and assessed their individual and combined effects in

an in vitro osteoporosis model. Extracts with varying leaf-to-fruit ratios (SH82, SH55,

SH28) were evaluated in MC3T3-E1 and RAW264.7 cells to examine osteogenesis and bone

resorption biomarkers. SH leaves were rich in chlorogenic acids (CGAs) and flavonoids,

while fruits contained phenolic acids with minimal flavonoids. Leaf extracts exhibited

superior antioxidant activity and ROS suppression. Both leaf and fruit extracts enhanced

ALP activity, calcium deposition, and collagen formation in MC3T3-E1 cells, with leaf extracts

demonstrating greater efficacy. Additionally, osteoblastogenesis-related genes were

upregulated, while TRAP activity and osteoclast-related gene expression were significantly

inhibited. The combined extract exerted a synergistic effect, with SH28 showing the most

pronounced osteogenic enhancement and TRAP inhibition. Key components, including

neo-CGA, CGA, rutin, and luteolin-7-O-glucoside, positively influenced ALP and TRAP

activities. These findings highlight the potential of SH, particularly at a high leaf-to-fruit

ratio, as a promising natural agent for osteoporosis prevention.